The Nucleus
The Nucleus
The Nucleus (Methods in Molecular Biology) by Ronald Hancock
This volume presents detailed,
recently-developed protocols ranging from isolation of nuclei to
purification of chromatin regions containing single genes, with a
particular focus on some less well-explored aspects of the nucleus. The
methods described include new strategies for isolation of nuclei, for
purification of cell type-specific nuclei from a mixture, and for rapid
isolation and fractionation of nucleoli. For gene delivery into and
expression in nuclei, a novel gentle approach using gold nanowires is
presented. As the concentration and localization of water and ions are
crucial for macromolecular interactions in the nucleus, a new approach
to measure these parameters by correlative optical and cryo-electron
microscopy is described. The Nucleus, Second Edition presents
methods and software for high-throughput quantitative analysis of 3D
fluorescence microscopy images, for quantification of the formation of
amyloid fibrils in the nucleus, and for quantitative analysis of
chromosome territory localization. Written in the successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls.
Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
Ebook format: PDFAuthoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
Ebook page: 251
File size: 8.92 MB
$55.00
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