Pyrosequencing: Methods and Protocols
Pyrosequencing: Methods and Protocols
Pyrosequencing: Methods and Protocols (Methods in Molecular Biology) by Ulrich Lehmann and Jörg Tost
The primary purpose of this volume is to
demonstrate the range of applications of the Pyrosequencing technology
in research and diagnostics and to provide detailed protocols. Beginning
with an up-to-date overview of the biochemistry, the volume continues
with quantitative analysis of genetic variation, ratio of expressed
alleles at the RNA level, analysis of DNA methylation, global DNA
methylation assays, specialized applications for DNA methylation
analysis including loss of imprinting, single blastocyst analysis,
allele-specific DNA methylation patterns, DNA methylation patterns
associated with specific histone modifications. The volume further
details tools and protocols for the detection of viruses and bacteria,
and genetic and epigenetic analyses for forensics using Pyrosequencing.
As a volume in the highly successful Methods in Molecular Biology
series, chapters contain introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible protocols and tips on troubleshooting and avoiding known
pitfalls.
Comprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.
Ebook format: PDFComprehensive and adaptable, Pyrosequencing: Methods and Protocols, Second Edition will greatly aid doctorial students, postdoctoral investigators and research scientists studying different aspects of genetics and cellular and molecular biology.
Ebook page: 429
File size: 12.57 MB
$40.00
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